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Cross reactivity antibody3/2/2024 ![]() This phenomenon can cause cross-reactivity and unexpected interactions. When an antibody deployed against one specific antigen has a competing affinity toward another antigen, it can recognize two different antigens with similar structural regions. However, they are more susceptible to cross-reactivity due to their ability to bind with multiple epitopes and the production of different clones of plasma B cells. What Is Cross-Reactivity in an Immunoassay?įor these reasons, polyclonal antibodies are the recommended capture antibody in sandwich ELISA tests. Polyclonal antibodies are often faster at capturing of target proteins and binding to the antigen, and less vulnerable to changes from pH, buffer, and storage conditions. This means polyclonal antibodies are more sensitive, as well as more robust for detecting small variations in individual isotopes of proteins. Polyclonal antibodies are utilized more often for general applications because they are a heterogenous antibody population capable of interacting with different epitopes on the same antigen. Researchers can use polyclonal or monoclonal antibodies. Each antibody features a specific amino acid sequence that dictates its affinity to a corresponding antigen. This is what makes them a valuable tool for a wide variety of research applications that involve identifying and detecting target proteins in immunoassays. ![]() Antibodies show a strong affinity to specific sequences on antigens.
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